In-vitro Screening for Disease Resistance in Wheat Genotypes against Bipolaris sorokiniana Using Callus Culture Method

Abstract

The present investigation was done to identify the efficacy of callus culture method for in-vitro screening to identify resistant and susceptible wheat genotypes. The study led to establishment of protocol for in-vitro screening of susceptible and resistant wheat genotypes against Bipolaris sorokiniana. The Bipolaris sorokiniana crude toxin was used at various concentrations to supplement the callusing medium and the response of twelve genotypes was studied. The susceptible genotype Agra local showed maximum area prone to death of the callus because of effect of toxin in medium supplemented with MS+2,4-D (4.0 mgl-1) + NAA (2 mgl-1) with B. sorokiniana toxin whereas the resistant genotype Yangmai#6 showed least area affected by the toxin and the cream/ white callus observed in the case of controlled medium supplemented with toxin. The genotypes which were found to be resistant and susceptible in the field condition were clearly identified as the same using this method.